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    现在位置首页>实验试剂制品>RNAi技术>siRNA转染> SiRNA的高效转染试剂——GeneSilencer Reagent

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    SiRNA的高效转染试剂——GeneSilencer Reagent

    价    格:询价

    产    地:美国更新时间:2019/7/15 13:41:34

    品    牌:Genlantis型    号:

    状    态:正常点击量:1946

    15665882320
    联系我时,请说明是在来宝网上看到的,谢谢!

    济南济威生物技术有限公司

    联 系 人:张经理

    电     话:0531-81307925

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    等     级: (第 1年)

    性     质:生产型,

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    Genlantis公司的专业SiRNA公司转染试剂,具有以下优势:

    最高转染效率的SiRNA的转染试剂

    最低细胞毒性的SiRNA的转染试剂

    最有效的功能基因沉默后siRNA的转染试剂

    可以应用于不同有无血清培养条件的细胞的转染

    拥有最简易的针对各种贴壁细胞和悬浮细胞转染的简易高效转染流程

    是目前用于哺乳动物细胞SiRNA转染最有效的试剂

    ?

    产品信息:

    供应商

    目录号

    产品说明

    规格

    价格

    特价

    Genlantis

    T500020

    GeneSilencer siRNA Transfection Reagent

    For up to 56 transfections in 24-well plates,up to 200 transfections in 96-well plates

    1352

    946

    Genlantis

    T500750

    GeneSilencer siRNA Transfection Reagent

    For up to 224 transfections in 24-well plates,up to 800 transfections in 96-well plates

    4438

    3107

    Genlantis

    T505750

    GeneSilencer siRNA Transfection Reagent

    For up to 1120 transfections in 24-well plates,up to 4000 transfections in 96-well plates

    17394

    12176

    7折促销,促销日期为:20106120101231

    ?

    siRNA的转染是基因沉默实验中关键的一步。GeneSilencer转染试剂是一种专为SiRNA转染设计的新型、高效率的脂质体阳离子转染试剂,专门用于siRNA转染,确保没有RNAse活性。传统的转染试剂有?#27426;?#30340;细胞毒性,在转染过程由于提高细胞的通透性因而不能在培养基中添加抗生素。由于GeneSilencer的内毒素和细胞毒性都非常之低,加上转染复合物能够高效的在完全培养基中形成,因而整个转染过程可以在含血清而且是含有抗生素的培养基中操作,无需更换培养基,这极大的方便转染操作!

    ?

    该试剂可以耐受较高浓度的siRNA转染而不引起细胞毒性,能够得到更好的抑制效果,适用于包括Aortic Muscle CellsCerebellar NeuronsHippocampal NeuronsOsteoblastAveolar Type II CellsCoronary Artery EndothelialHMVECPulmonary Artery Endothelium Bone MarrowCortical NeuronsLacrimal Gland Acinar CellsRetinal GanglionCardiac FibroblastDendritic CellsLung Microvascular Endothelial CellsSchwann CellsCardiac MyocytesDorsal Root Ganglion NeuronsMacrophageT-Cells (CD3+).在内的多种真核细胞。

    ?

    GeneSilencer能对多种细胞类型的SiRNA进行非常有效的转染。下面是部分细胞系SiRNA成功转染的相关?#21335;住?#20247;多的?#21335;?#25253;道该转染试剂已经成为SiRNA转染的潮流与首选。?

    Cell Line

    Cell Type

    Source

    Citation

    Primary

    Aortic Muscle Cells

    Rat

    Lake, A.C. & Castellot, J.J. (2003) CCN5 modulates the antiproliferative effect of heparin and regulates cell motility in vascular smooth muscle cells. Cell Comm. &Signaling 1: 5.

    Primary

    Aveolar Type II Cells

    Mouse

    Ueno, T., Linder, S., Na, C-L, Rice, W.R., Johansson, J., and Weaver, T.E. (2004) Processing of Pulmonary Surfactant Protein B by Napsin and Cathepsin H. J. Biol.Chem. 279: 16178-16184.

    Primary

    Bone Marrow

    Mouse

    Yang, R., Cai, Z., Zhang, Y., Yutzy IV, W.H., Roby, K.F. and Roden, R.B.S. (2006) CD80 in Immune Suppression by Mouse Ovarian Carcinoma?Associated Gr-1+CD11b+ Myeloid Cells. Cancer Res 66 (13): 6807 - 6815.

    Primary

    Cardiac Fibroblast

    Mouse

    Hu, C., Dandapat, A., Sun, L., Khan, J.A., Liu, Y., Hermonat, P.L. and Mehta, J.L. (2008) Regulation of TGFbeta 1-mediated collagen formation by LOX-1: Studies based on forced over-expression of TGFbeta 1 in wild-type and LOX-1 knockout mice cardiac fibroblasts. J. Biol. Chem. 283 (16) 10226-10231.

    Primary

    Cardiac Myocytes

    Feline

    Ramabadran, R. S., Chancey, A., Vallejo, J.G., Barger, P.M., Sivasubramanian, N. and Mann, D.L. (2008) Targeted Gene Silencing of Tumor Necrosis Factor Attenuates the Negative Inotropic Effects of Lipopolysaccharide in Isolated Contracting Cardiac Myocytes. Tex Heart Inst J. 35(1): 16-21.

    Primary

    Cardiac Myocytes(Neonatal)

    Rat

    Juhaszova, M., Zorov, D.B., Kim, S-H, Pepe, S., Fu, O., Fishbein, K.W., Ziman, B.D., Wang, S., Ytrehus, K., Antos, C.L., Olson, E.N. and Sollott, S.J. (2004) Glycogen synthase kinase-3{beta} mediates convergence of protection signaling to inhibit the mitochondrial permeability transition pore. J. Clin. Invest. 113(11): p. 1535-1549.

    Primary

    Cerebellar Neurons Coronary Artery Endothelial

    Rat Human HCAEC

    Numakawa, T., Nakayama, H., Suzuki, S., Kubo, T., Nara, F., Numakawa, Y., Yokomaku, D., Araki, T., Ishimoto, T., Ogura, A. and Taguchi, T. (2003) Nerve growth factor-induced glutamate release is via p75 receptor, ceramide and Ca2+ from ryanodine receptor in developing cerebellar neurons. J. Biol. Chem: 278: 41259-41269. Dandapat, A., Hu, C., Sun, L. and Mehta, J.L. (2007) Small Concentrations of oxLDLInduce Capillary Tube Formation From Endothelial Cells via LOX-1 Dependent Redox-Sensitive Pathway. Arterioscler Thromb Vasc Biol.

    Primary

    Cortical Neurons

    Mouse

    Aarts, M., Lihara, K., Wei, W-L, Xiong, Z-G, Arundine, M., Cerwinski, W., MacDonald, J.F., Tymianski, M. (2003) A Key Role for TRPM7 Channels in Anoxic Neuronal Death. Cell 115: 863.

    Primary

    Cortical Neurons

    Mouse

    Aleyasin, H., Cregan, S.P., Iyirhiaro, G., OHare, M.J., Callaghan, S.M., Slack, R.S. and Park, D.S. (2004) Nuclear Factor-B Modulates the p53 Response in Neurons Exposed to DNA Damage. J. Neurosci. 24 (12): 2963-2973.

    Primary

    Cortical Neurons

    Mouse

    Burkhalter, J., Fiumelli, H., Erickson, J.D. and Martin, J-L. (2007) A critical role for system a amino acid transport in the regulation of dendritic development by BDNF. J Biol Chem 282: 5152 - 5159.

    Primary

    Cortical Neurons

    Mouse

    Cui, H., Hayashi, A., Sun, H-S, Belmares, M.P., Cobey, C., Phan, T., Schweizer, J., Salter, M.W., Wang, Y.T., Tasker, R.A., Garman, D., Rabinowitz, J., Lu, P.S. and Tymianski, M. (2007) PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors. J. Neurosci.27(37): 9901-9915.

    Primary

    Cortical Neurons

    Mouse

    Sato, S., Xu, J., Okuyama, S., Martinez, LB., Walsh, S.M, Jacobsen, M.T, Swan, R.J., Schlautman, J.D., Ciborowski, P. and Ikezu, T. (2008) Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1. J Neurosci 28(53): 14511.





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