Cathepsin S is a cysteine protease of the papain family, found inside the lysosomal/endosomal compartments of antigen-presenting cells such as B cells, macrophages and dendritic cells. Cathepsin S participates in the degradation of antigenic proteins to peptides for presentation to the MHC class II. Cathepsin S has additional biological effects, acting as elastase on the surface of vascular endothelial cells, a process that contributes to microvascular complications in diabetes mellitus.
The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well format, with purified Cathepsin S, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions. In addition, the kit includes the cathepsin inhibitor E64 for use as a control inhibitor.
Materials Required But Not Provided:
? 0.5 M DTT in aqueous solution
? Adjustable micropipettor and sterile tips
? Fluorescent microplate reader
Cathepsin S, CTSS, activity assay kit
The Cathepsin S assay kit comes in a convenient 96-well format, with purified Cathepsin S, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions.
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Tato, M., et al. (2017). Cathepsin S inhibition combines control of systemic and peripheral pathomechanisms of autoimmune tissue injury. Scientific reports 7(1), 2775