The BCL2A1 TR-FRET Assay Kit is designed to measure the inhibition of BCL2A1 (BFL1) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2A1, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Materials Required But Not Provided:
Dithiothreitol (DTT, 0.5 M) Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips
BCL2L5, BCL2-L5, BFL1, GRS, HBPA1, HTRF
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Kvansakul, M., Hinds, MG. Cell Death and Disease 2013; 4: e909.