The BCL-2 TR-FRET Assay Kit is designed to measure the inhibition of BCL-2 binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbiumlabeled donor, dye-labeled acceptor, BCL-2, peptide ligand, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Bcl, Bcl2, B cell lymphoma 2, HTRF, BCLXL, BCL2
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
At least 6 months from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Filippakopoulos, P., et al.,Cell 2012; 149:214.
Since January of 2018, this kit has been optimized for better performance. The previous version of the BCL-2 TR-FRET Assay Kit, Cat. #50222, can still be purchased upon special request.
Avoid freeze/thaw cycles.